Antimicrobial Effect of Different Intracanal Dressings in Endodontic Treatment

Na četiri hranilišta kontaktnim testom in vitro ispitan je utjecaj pet različitih intrakanalnih medikamentoznih uložaka: kalcij-hidroksida, klorheksidin glukonata u obliku gela 1% i otopine 0,2%, paraklorfenolkamfora i 1% metronidazola. Mikroorganizmi kojima se arteficijalno inficirao korijenski kanal 40 ispitanih ekstrahiranih zuba bile su: Pseudomonas aeruginosa, Staphylococcus aureus i Candida albicans. Rezultati su pokazali znatne razlike među utjecajima pet ispitanih intrakanalnih uložaka na brojnost mikroorganizama P. aeruginosa nakon tri i sedam dana (p < 0,05) te C. albicans nakon tri dana (p < 0,05). Preparati na temelju kalcij-hidroksida i paraklorfenolkamfora imaju veliku antimikrobnu učinkovitost. Paraklorfenolkamfor je znatno reducirao broj svih ispitanih mikroorganizama P. aeruginosa i C. albicans, nakon tri i nakon sedam dana u usporedbi s ostalim materijalima (p < 0,05). Kalcijev hidroksid je pokazao isti učinak na P. aeruginosa kao i paraklorfenomkamfor, s obzirom na to da je djelovao nakon tri dana (p < 0,05). Klorheksidin gel je imao najslabije antibakterijsko djelovanje na P. aeruginosa nakon tri dana u odnosu prema svim ispitanim materijalima, ali njegovo antibakterijsko djelovanje nakon sedam dana bilo je statistički znatno veće u usporedbi s ostalim materijalima (p < 0,05), osim paraklorfenokamfora. Svi testirani materijali imali su dobar antimikotski učinak na C. albicans, osim metronidazola koji - kao što se i očekivalo - uopće nije djelovao na gljivu (p < 0,05). Može se zaključiti da klorheksidin gel povećava antibakterijsko djelovanje s duljinom primjene, a paraklorfenolkamfor i kalcij-hidroksid pokazuju najbrže i najjače antimikrobno djelovanje.The effect of five intracanal dressings, calcium hydroxide, chlorhexidine gluconate (1% gel and 0.2% solution), camphorated parachlorphenole and 1% metronidazole was tested in vitro in four media. The canals of 40 test teeth were artificially infected with Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans. Significant differences were observed between five intracanal dressings regarding the number of P. aeruginosa after 3 and 7 days (p < 0.05) and C. albicans after 3 days (p < 0.05). Specimens with calcium hydroxide and camphorated parachlorphenole have significant antibacterial efficacy. Camphorated parachlorphenole has significantly reduced the number of all tested microorganisms P. aeruginosa and C. albicans after 3 and 7 days in comparison with other materials (p < 0.05). Calcium hydroxide has shown similar effect on P. aeruginosa as camphorated parachlorphenole regarding the efficacy after 3 days (p < 0.05). Chlorhexidine gel has shown the weakest efficacy on P. aeruginosa after 3 days, compared to all tested materials, but its antibacterial efficacy after 7 days was statistically greater compared to other materials (p < 0.05), except for camphorated parachlorphenole. All tested materials had good antimycotic efficacy against C. albicans except metronidazole that, as was expected, had no efficacy against yeast (p < 0.05). We can conclude that the antibacterial efficacy of chlorhexidine gel is enhanced with time, while camphorated parachlorphenole and calcium hydroxide have fastest and strongest antimicrobial efficacy

Antimicrobial Effect of Different Intracanal Dressings in Endodontic Treatment

Na četiri hranilišta kontaktnim testom in vitro ispitan je utjecaj pet različitih intrakanalnih medikamentoznih uložaka: kalcij-hidroksida, klorheksidin glukonata u obliku gela 1% i otopine 0,2%, paraklorfenolkamfora i 1% metronidazola. Mikroorganizmi kojima se arteficijalno inficirao korijenski kanal 40 ispitanih ekstrahiranih zuba bile su: Pseudomonas aeruginosa, Staphylococcus aureus i Candida albicans. Rezultati su pokazali znatne razlike među utjecajima pet ispitanih intrakanalnih uložaka na brojnost mikroorganizama P. aeruginosa nakon tri i sedam dana (p < 0,05) te C. albicans nakon tri dana (p < 0,05). Preparati na temelju kalcij-hidroksida i paraklorfenolkamfora imaju veliku antimikrobnu učinkovitost. Paraklorfenolkamfor je znatno reducirao broj svih ispitanih mikroorganizama P. aeruginosa i C. albicans, nakon tri i nakon sedam dana u usporedbi s ostalim materijalima (p < 0,05). Kalcijev hidroksid je pokazao isti učinak na P. aeruginosa kao i paraklorfenomkamfor, s obzirom na to da je djelovao nakon tri dana (p < 0,05). Klorheksidin gel je imao najslabije antibakterijsko djelovanje na P. aeruginosa nakon tri dana u odnosu prema svim ispitanim materijalima, ali njegovo antibakterijsko djelovanje nakon sedam dana bilo je statistički znatno veće u usporedbi s ostalim materijalima (p < 0,05), osim paraklorfenokamfora. Svi testirani materijali imali su dobar antimikotski učinak na C. albicans, osim metronidazola koji - kao što se i očekivalo - uopće nije djelovao na gljivu (p < 0,05). Može se zaključiti da klorheksidin gel povećava antibakterijsko djelovanje s duljinom primjene, a paraklorfenolkamfor i kalcij-hidroksid pokazuju najbrže i najjače antimikrobno djelovanje.The effect of five intracanal dressings, calcium hydroxide, chlorhexidine gluconate (1% gel and 0.2% solution), camphorated parachlorphenole and 1% metronidazole was tested in vitro in four media. The canals of 40 test teeth were artificially infected with Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans. Significant differences were observed between five intracanal dressings regarding the number of P. aeruginosa after 3 and 7 days (p < 0.05) and C. albicans after 3 days (p < 0.05). Specimens with calcium hydroxide and camphorated parachlorphenole have significant antibacterial efficacy. Camphorated parachlorphenole has significantly reduced the number of all tested microorganisms P. aeruginosa and C. albicans after 3 and 7 days in comparison with other materials (p < 0.05). Calcium hydroxide has shown similar effect on P. aeruginosa as camphorated parachlorphenole regarding the efficacy after 3 days (p < 0.05). Chlorhexidine gel has shown the weakest efficacy on P. aeruginosa after 3 days, compared to all tested materials, but its antibacterial efficacy after 7 days was statistically greater compared to other materials (p < 0.05), except for camphorated parachlorphenole. All tested materials had good antimycotic efficacy against C. albicans except metronidazole that, as was expected, had no efficacy against yeast (p < 0.05). We can conclude that the antibacterial efficacy of chlorhexidine gel is enhanced with time, while camphorated parachlorphenole and calcium hydroxide have fastest and strongest antimicrobial efficacy